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ProductsBack/Goat anti-T-cell differentiation protein

Goat anti-T-cell differentiation protein

EB07850
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetMAL
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-T-cell differentiation protein Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID4118
  • Target name
    MAL
  • Target description
    mal, T cell differentiation protein (MAL blood group)
  • Target synonyms
    HLD28, MVP17, VIP17, myelin and lymphocyte protein, T-lymphocyte maturation-associated protein, mal, T-cell differentiation protein
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_002362.1; NP_071884.1. GeneIDs all Nonhuman: 17153 (mouse); 25263 (rat);. Purification: Antigen affinity purified. Names and symbols: MAL; mal, T-cell differentiation protein ; T-cell differentiation protein MAL; T-lymphocyte maturation-associated protein; myelin and lymphocyte protein
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MAL using anti-MAL antibody (A06941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human HL-60 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAL antigen affinity purified polyclonal antibody (Catalog # A06941-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MAL at approximately 17KD. The expected band size for MAL is at 17KD.
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