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ProductsBack/Goat anti-TNF-R-II / TNFR2 (mouse) Antibody

Goat anti-TNF-R-II / TNFR2 (mouse) Antibody

EB11050
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTnfrsf1b
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TNF-R-II / TNFR2 (mouse) Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID21938
  • Target name
    Tnfrsf1b
  • Target description
    tumor necrosis factor receptor superfamily, member 1b
  • Target synonyms
    CD120b, TNF-R-II, TNF-R2, TNF-R75, TNF-alphaR2, TNFBR, TNFR80, TNFRII, TNFalpha-R2, Tnfr-1, Tnfr2, p75, tumor necrosis factor receptor superfamily member 1B, TNF receptor beta chain, TNF-RII, TNFR-II, p75 TNFR, p80 TNF-alpha receptor, tumor necrosis factor receptor 2, tumor necrosis factor receptor type II
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_035740.2. GeneIDs all Nonhuman: 21938 (mouse); 156767 (rat);. Purification: Antigen affinity purified. Names and symbols: Tnfrsf1b; tumor necrosis factor receptor superfamily, member 1B; CD120b; TBPII; TNF-R-II; TNF-R75; TNFBR; TNFR1B; TNFR2; TNFR80; p75; p75TNFR; TNF-R2; TNF-RII; TNFR-II; p75 TNF receptor; p80 TNF-alpha receptor; soluble TNFR1B variant 1; tumor necrosis fac
  • Reactivity
    Human, Mouse, Rat
  • Reactivity Supplier
    Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNF Receptor II using anti-TNF Receptor II antibody (PB9515). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Thymus Tissue Lysate at 50ug, Lane 2: Mouse Thymus Tissue Lysate at 50ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNF Receptor II antigen affinity purified polyclonal antibody (Catalog # PB9515) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNF Receptor II at approximately 75 kDa. The expected band size for TNF Receptor II is at 75 kDa.
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