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Goat anti-TNNT3

EB12033
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNNT3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-TNNT3 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7140
  • Target name
    TNNT3
  • Target description
    troponin T3, fast skeletal type
  • Target synonyms
    beta-TnTF; DA2B2; TNTF; troponin T type 3 (skeletal, fast); troponin T, fast skeletal muscle
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_006748.1; NP_001036246.1; NP_001036247.1. GeneIDs all Nonhuman: 21957 (mouse); 24838 (rat);. Purification: Antigen affinity purified. Names and symbols: TNNT3; troponin T type 3 (skeletal, fast); TNTF; beta TnTF; beta-TnTF; fTnT; fast skeletal muscle troponin T; troponin T, fast skeletal muscle; troponin-T3, skeletal, fast
  • Reactivity Supplier
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TNNT3 using anti-TNNT3 antibody (A05885-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle issue lysates, Lane 2: mouse skeletal muscle issue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TNNT3 antigen affinity purified polyclonal antibody (Catalog # A05885-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TNNT3 at approximately 37KD. The expected band size for TNNT3 is at 32KD.
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