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Goat anti-UNC5C

EB09218
Everest Biotech
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUNC5C
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-UNC5C Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Staining of paraffin embedded endocrine islets (red) in adult Mouse Pancreas. Nuclear staining in blue and ductal cell staining in green. Data obtained from a customer.
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, IF, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID8633
  • Target name
    UNC5C
  • Target description
    unc-5 netrin receptor C
  • Target synonyms
    netrin receptor UNC5C; protein unc-5 homolog 3; protein unc-5 homolog C; unc5 (C.elegans homolog) c; unc-5 homolog 3; unc-5 homolog C; UNC5H3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_003719.2. GeneIDs all Nonhuman: 22253 (mouse);. Purification: Antigen affinity purified. Names and symbols: UNC5C; unc-5 homolog C (C. elegans); UNC5H3; homolog of C. elegans transmembrane receptor Unc5; unc5 (C.elegans homolog) c; unc5C
  • Reactivity Supplier
    Human, Mouse, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UNC5C using anti-UNC5C antibody (PB9841). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UNC5C antigen affinity purified polyclonal antibody (Catalog # PB9841) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UNC5C at approximately 115 kDa. The expected band size for UNC5C is at 115 kDa.
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