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Goat anti-WNT3 Antibody

EB07253
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetWNT3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-WNT3 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID7473
  • Target name
    WNT3
  • Target description
    Wnt family member 3
  • Target synonyms
    INT4, TETAMS, proto-oncogene Wnt-3, WNT-3 proto-oncogene protein, proto-oncogene Int-4 homolog, wingless-type MMTV integration site family, member 3
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_110380.1. GeneIDs all Nonhuman: 22415 (mouse); 24882 (rat);. Purification: Antigen affinity purified. Names and symbols: WNT3 ; wingless-type MMTV integration site family, member 3 ; INT4; MGC131950; MGC138321; MGC138323 ; WNT-3 proto-oncogene protein precursor
  • Reactivity
    Canine, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Wakabayashi T, Hidaka R, Fujimaki S, et al. Diabetes Impairs Wnt3 Protein-induced Neurogenesis in Olfactory Bulbs via Glutamate Transporter 1 Inhibition. J Biol Chem. 2016,291(29):15196-211. doi: 10.1074/jbc.M115.672857
    Read this paper
  • Fujimaki S, Hidaka R, Asashima M, et al. Wnt protein-mediated satellite cell conversion in adult and aged mice following voluntary wheel running. J Biol Chem. 2014,289(11):7399-412. doi: 10.1074/jbc.M113.539247
    Read this paper
  • Okamoto M, Inoue K, Iwamura H, et al. Reduction in paracrine Wnt3 factors during aging causes impaired adult neurogenesis. FASEB J. 2011,25(10):3570-82. doi: 10.1096/fj.11-184697
    Read this paper

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Figure 1. Western blot analysis of WNT3 using anti-WNT3 antibody (PB9619). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Human Placenta Tissue Lysate at 50ug, Lane 2: HEPG2 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WNT3 antigen affinity purified polyclonal antibody (Catalog # PB9619) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WNT3 at approximately 45 kDa. The expected band size for WNT3 is at 45 kDa.
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