Western Blot of HRP conjugated anti-Goat IgG antibody R1318HRP showing detection of 50ng of Goat IgG (Lane 1) but not Human IgG (Lane 2). Samples were separated by 4-20% SDS-PAGE under reducing conditions and transferred to nitrocellulose membrane. The blot was blocked overnight at 4°C in 5% BSA in TBS. A 1/5,000 dilution of antibody in Blocking Buffer for Fluorescent Western Blotting was used to probe the membrane at RT for 1 h. The image was developed using Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate (p/n Femtomax-020) for one minute.