ELISA Results of Chicken Anti-Goat IgG Antibody Alkaline Phosphatase Conjugated tested against purified Goat IgG. Each well was coated in duplicate with 1.0 µg of Goat IgG (p/n 005-0102). The working dilution of Goat IgG Alk Phos is 1:3700. The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP stabilizer (p/n MB-067) and NPP Buffer (p/n NPP-500).