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ProductsBack/GOLGA1 / Golgin-97 Antibody

GOLGA1 / Golgin-97 Antibody

LS-C749672
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGOLGA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GOLGA1 / Golgin-97 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 88kDa, while the observed MW by Western blot was 105kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 88kDa, while the observed MW by Western blot was 105kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2800
  • Target name
    GOLGA1
  • Target description
    golgin A1
  • Target synonyms
    golgin-97, golgin subfamily A member 1, gap junction protein, alpha 4, 37kD, golgi autoantigen, golgin subfamily a, 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Golgin 97 antibody [C2C3], C-term (GTX114445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of Golgin 97/GOLGA1 using anti-Golgin 97/GOLGA1 antibody (A13524-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Golgin 97/GOLGA1 antigen affinity purified polyclonal antibody (Catalog # A13524-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Golgin 97/GOLGA1 at approximately 88-97 kDa. The expected band size for Golgin 97/GOLGA1 is at 88 kDa.
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