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ProductsBack/GOLM1 / GP73 / GOLPH2 Antibody (clone 5B4-G6-C9-C6-G4, Preservative Free)

GOLM1 / GP73 / GOLPH2 Antibody (clone 5B4-G6-C9-C6-G4, Preservative Free)

LS-C343204
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetGOLM1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GOLM1 / GP73 / GOLPH2 Antibody (clone 5B4-G6-C9-C6-G4, Preservative Free)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:320000), WB (1:1000 - 1:5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5B4-G6-C9-C6-G4
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51280
  • Target name
    GOLM1
  • Target description
    golgi membrane protein 1
  • Target synonyms
    C9orf155, GOLPH2, GP73, HEL46, PSEC0257, bA379P1.3, Golgi membrane protein 1, epididymis luminal protein 46, golgi membrane protein GP73, golgi phosphoprotein 2, golgi protein, 73-kD
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GOLPH2/GOLM1 using anti-GOLPH2/GOLM1 antibody (A02975-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GOLPH2/GOLM1 antigen affinity purified polyclonal antibody (Catalog # A02975-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GOLPH2/GOLM1 at approximately 73 kDa. The expected band size for GOLPH2/GOLM1 is at 73 kDa.
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