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Western Blot analysis of K562 cells using Glypican-5 Polyclonal Antibody
Western Blot analysis of K562 cells using Glypican-5 Polyclonal Antibody
Western Blot analysis of K562 cells using Glypican-5 Polyclonal Antibody

GPC5 Antibody

CSB-PA008249
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGPC5
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Overview

  • Supplier
    Cusabio
  • Product Name
    GPC5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2262
  • Target name
    GPC5
  • Target description
    glypican 5
  • Target synonyms
    bA93M14.1; glypican proteoglycan 5; glypican-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78333
  • Protein Name
    Glypican-5
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Glypican 5/GPC5 using anti-Glypican 5/GPC5 antibody (A07532-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U87 whole cell lysates, Lane 3: human U20S whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Glypican 5/GPC5 antigen affinity purified polyclonal antibody (Catalog # A07532-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Glypican 5/GPC5 at approximately 64KD. The expected band size for Glypican 5/GPC5 is at 64KD.
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