More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GPHN Polyclonal Antibody

GPHN Polyclonal Antibody

RD85189A
Reddot Biotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGPHN
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    GPHN Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10243
  • Target name
    GPHN
  • Target description
    gephyrin
  • Target synonyms
    GEPH; gephyrin; GPH; GPHRYN; HKPX1; MOCODC
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a neuronal assembly protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton via high affinity binding to a receptor subunit domain and tubulin dimers. In nonneuronal tissues, the encoded protein is also required for molybdenum cofactor biosynthesis. Mutations in this gene may be associated with the neurological condition hyperplexia and also lead to molybdenum cofactor deficiency. Numerous alternatively spliced transcript variants encoding different isoforms have been described; however, the full-length nature of all transcript variants is not currently known.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
Gephyrin Monoclonal AntibodyCAB4729
TargetGPHN
  • SizePrice
Product group Antibodies
References
GeneTex
Gephyrin antibody [N2C1], InternalGTX109734
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetGPHN
  • SizePrice
Product group Antibodies
MedChem Express
Gephyrin Antibody (YA2246)HY-P82501
TargetGPHN
  • SizePrice
IHC image of CSB-PA009716LA01HU diluted at 1:300 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
GPHN AntibodyCSB-PA009716LA01HU
ApplicationsELISA, ImmunoHistoChemistry
TargetGPHN
  • SizePrice
Product group Antibodies
Biosynth
Gephyrin antibody70R-2208
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-Gephyrin Antibody, Affinity PurifiedA304-245A
ApplicationsImmunoPrecipitation, Western Blot
TargetGPHN
  • SizePrice
Product group Antibodies
RayBiotech
Anti-GPHN Antibody144-08572
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetGPHN
  • SizePrice
Figure 1. Western blot analysis of Gephyrin/GPHN using anti-Gephyrin/GPHN antibody (A04560-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: human Jurkat whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse kidney tissue lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gephyrin/GPHN antigen affinity purified polyclonal antibody (Catalog # A04560-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gephyrin/GPHN at approximately 93 kDa. The expected band size for Gephyrin/GPHN is at 93 kDa.
Product group Antibodies
Boster Bio
Anti-Gephyrin/GPHN Antibody Picoband(r)A04560-1-CARRIER-FREE
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetGPHN
  • SizePrice
Product group Antibodies
Bioss Antibodies
Gephyrin Recombinant Antibody, AbBy Fluor-647 ConjugatedBSM-61891R-BF647
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetGPHN
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
Gephyrin AntibodyLS-C403177
ApplicationsELISA, ImmunoHistoChemistry
TargetGPHN
  • SizePrice