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Western Blot Positive WB detected in: PC-3 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue, Mouse brain tissue All lanes: GPI antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64, 65 kDa Observed band size: 64 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue, Mouse brain tissue All lanes: GPI antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64, 65 kDa Observed band size: 64 kDa
Western Blot Positive WB detected in: PC-3 whole cell lysate, Hela whole cell lysate, Mouse kidney tissue, Mouse brain tissue All lanes: GPI antibody at 2.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 64, 65 kDa Observed band size: 64 kDa

GPI Antibody

CSB-PA00367A0RB
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetGPI
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Overview

  • Supplier
    Cusabio
  • Product Name
    GPI Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2821
  • Target name
    GPI
  • Target description
    glucose-6-phosphate isomerase
  • Target synonyms
    AMF, CNSHA4, GNPI, NLK, PGI, PHI, SA-36, SA36, glucose-6-phosphate isomerase, autocrine motility factor, hexose monophosphate isomerase, hexosephosphate isomerase, neuroleukin, oxoisomerase, phosphoglucose isomerase, phosphohexomutase, phosphohexose isomerase, phosphosaccharomutase, sperm antigen-36
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06744
  • Protein Name
    Glucose-6-phosphate isomerase
  • Scientific Description
    Besides its role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GPI using anti-GPI antibody (PB10068). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPI antigen affinity purified polyclonal antibody (Catalog # PB10068) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPI at approximately 63 kDa. The expected band size for GPI is at 63 kDa.
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