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Gpi Antibody

CSB-PA009717LA01MO
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityMouse
TargetGpi1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Gpi Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID14751
  • Target name
    Gpi1
  • Target description
    glucose-6-phosphate isomerase 1
  • Target synonyms
    Amf, Gpi, Gpi-1, Gpi-1r, Gpi-1s, Gpi-1t, Gpi1-r, Gpi1-s, Gpi1-t, Gpi1s, MF, NK, NK/GPI, Nlk, Org, Pgi, Phi, glucose-6-phosphate isomerase, autocrine motility factor, glucose phosphate isomerase 1 complex, glucose phosphate isomerase 1, regulatory, glucose phosphate isomerase 1, structural, glucose phosphate isomerase 1, temporal, maturation factor, neuroleukin, phosphoglucose isomerase, phosphohexose isomerase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06745
  • Protein Name
    Glucose-6-phosphate isomerase
  • Scientific Description
    Besides its role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GPI using anti-GPI antibody (PB10069). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse HEPA1-6 whole cell lysates, Lane 2: mouse NIH/3T3 whole cell lysates, Lane 3: mouse spleen tissue lysates, Lane 4: mouse kidney tissue lysates, Lane 5: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPI antigen affinity purified polyclonal antibody (Catalog # PB10069) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GPI at approximately 64 kDa. The expected band size for GPI is at 63 kDa.
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