Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR31 Polyclonal Antibody, Unconjugated (bs-13530R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR31 Polyclonal Antibody, Unconjugated (bs-13530R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
GPR31 Polyclonal Antibody
BS-13530R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGPR31
Overview
- SupplierBioss
- Product NameGPR31 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2853
- Target nameGPR31
- Target descriptionG protein-coupled receptor 31
- Target synonyms12-HETER, HETER, HETER1, 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor, 12-(S)-HETE acid receptor, 12-(S)-HETE receptor, 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor, GPR31/12-HETER, bA517H2.2 (G protein-coupled receptor 31), hydroxyeicosatetraenoic (HETE) acid receptor 1, probable G-protein coupled receptor 31
- HostRabbit
- IsotypeIgG
- Protein IDO00270
- Protein Name12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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