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Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR31 Polyclonal Antibody, Unconjugated (bs-13530R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR31 Polyclonal Antibody, Unconjugated (bs-13530R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR31 Polyclonal Antibody, Unconjugated (bs-13530R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

GPR31 Polyclonal Antibody

BS-13530R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGPR31
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GPR31 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2853
  • Target name
    GPR31
  • Target description
    G protein-coupled receptor 31
  • Target synonyms
    12-(S)-HETE acid receptor; 12-(S)-HETE receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid (HETE) receptor; 12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor; 12-HETER; bA517H2.2 (G protein-coupled receptor 31); GPR31/12-HETER; HETER; HETER1; hydroxyeicosatetraenoic (HETE) acid receptor 1; probable G-protein coupled receptor 31
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00270
  • Protein Name
    12-(S)-hydroxy-5,8,10,14-eicosatetraenoic acid receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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