WB analysis of U87MG cells with/without GPX4 knockdown treatment using GTX03194 GPX4 antibody [GT1282]. Dilution : 1:1000 Loading : 25microg per lane
![WB analysis of various samples using GTX03194 GPX4 antibody [GT1282]. Dilution : 1:1000 Loading : 25microg per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX03194/GTX03194_4_WB_w_23053123_641.webp "WB analysis of various samples using GTX03194 GPX4 antibody [GT1282]. Dilution : 1:1000 Loading : 25microg per lane")
![Various whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX4 antibody [GT1282] (GTX03194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03194/GTX03194_4000000558_20210709_WB_w_23053123_755.webp "Various whole cell extracts (30 microg) were separated by 12% SDS-PAGE, and the membrane was blotted with GPX4 antibody [GT1282] (GTX03194) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of U87MG cells with/without GPX4 knockdown treatment using GTX03194 GPX4 antibody [GT1282]. Dilution : 1:1000 Loading : 25microg per lane
GPX4 antibody [GT1282]
GTX03194
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGPX4
Overview
- SupplierGeneTex
- Product NameGPX4 antibody [GT1282]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500 - 1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1282
- Concentration0.57 mg/ml
- ConjugateUnconjugated
- Gene ID2879
- Target nameGPX4
- Target descriptionglutathione peroxidase 4
- Target synonymsepididymis secretory sperm binding protein; GPx-4; GSHPx-4; MCSP; PHGPx; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; selenoprotein GPX4; SMDS; snGPx; snPHGPx; sperm nucleus glutathione peroxidase
- HostRabbit
- IsotypeIgG
- Protein IDP36969
- Protein NamePhospholipid hydroperoxide glutathione peroxidase
- Scientific DescriptionThe protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development; thus, it has been identified as a moonlighting protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3 UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2016]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203