GPX4 Fluorogenic Assay Kit

BPS Bioscience

GPX4 Fluorogenic Assay Kit

7

Research Use Only

The GPX4 Fluorogenic Assay Kit is a fluorogenic assay designed to measure GPX4 (glutathione peroxidase 4) activity for screening and profiling applications. The assay kit comes in a convenient 96-well format, with enough purified recombinant GPX4, Glutathione, Cumene Hydroperoxide, NADPH, Glutathione Reductase, and GPX4 Assay Buffer for 100 reactions. Figure 1: Mechanism of detection used in the GXP4 Assay Kit. GXP4 activity is measured indirectly. GXP4 converts reduced glutathione (GSH) to oxidized glutathione (GSSG) by reducing hydroperoxide (ROOH). GSSG is then converted back to its reduced form by glutathione reductase (GR) in the presence of NAPDH, which is converted into NAPD+. NADPH is intrinsically fluorescent, a property lost upon oxidation to NADP+. The decrease in fluorescence (exc lambda=340 nm; em lambda=450 nm) observed is proportional to the amount of GSSH available for conversion by GR, and to GXP4 activity.

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