GR-GAL4 Reporter (Luc)-HEK293 Recombinant Cell Line (Glucocorticoid Receptor Pathway)

BPS Bioscience

GR-GAL4 Reporter (Luc)-HEK293 Recombinant Cell Line (Glucocorticoid Receptor Pathway)

7

Research Use Only

The Glucocorticoid Receptor Pathway GAL4 Reporter (Luc) - HEK293 Cell Line contains a firefly luciferase gene under the control of glucocorticoid receptor ligand binding domain that is fused to the DNA binding domain (DBD) of GAL4 (GAL4 DBD-GR) stably integrated into HEK293 cells. This fusion construct activates firefly luciferase expression under the control of a multimerized GAL4 upstream activation sequence (UAS). This allows for specific detection of glucocorticoid-induced activation of the glucocorticoid receptor without the need for individual transcriptional targets and with low cross-reactivity for other nuclear receptor pathways. This cell line is validated for response to stimulation of dexamethasone and to the treatment with mifepristone, an inhibitor of the glucocorticoid signaling pathway.

Liquid Nitrogen

12352207