Grams Safranin Solution [477-73-6]

Chemodex

Grams Safranin Solution [477-73-6]

10

477-73-6

Research Use Only

Liquid

Warning

C20H19ClN4

350.84

Chemical. CAS: 477-73-6. Formula: C20H19ClN4. MW: 350.84. Synthetic. Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Grams crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange. - Gram Safranin Solution is a reagent recommended for use in qualitative procedures to differentiate gram-negative from gram-positive organisms. The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria walls is the basis of the color affinity. Bacteria will be stained with Grams crystal violet solution - an aniline dye - in the first step. After the treatment with iod solution, a dye-iod complex will form. During the decolorizing step, this complex stays in the multilayer mureine structures of the Gram-positive bacteria and they will appear blue. Gram-negative bacteria have a monolayer mureine structure only, the dye-iod complex does not stay bound to the cellwall, they will be decolorized. Gram-negative bacteria will be counterstained by safranin solution and appear orange.

CC1=C(N)C=C([N+](C2=CC=CC=C2)=C(C=C(N)C(C)=C3)C3=N4)C4=C1.[Cl-]

RT

41116134