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ProductsBack/GRASP65 (GORASP1) Mouse Monoclonal Antibody [Clone ID: OTI5G8]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GORASP1 (Cat# RC210076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1(Cat# TA501309). Positive lysates [LY403127] (100ug) and [LC403127] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GORASP1 (Cat# RC210076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1(Cat# TA501309). Positive lysates [LY403127] (100ug) and [LC403127] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GORASP1 (Cat# RC210076, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GORASP1(Cat# TA501309). Positive lysates [LY403127] (100ug) and [LC403127] (20ug) can be purchased separately from OriGene.

GRASP65 (GORASP1) Mouse Monoclonal Antibody [Clone ID: OTI5G8]

CF501309
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetGORASP1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    GRASP65 (GORASP1) Mouse Monoclonal Antibody [Clone ID: OTI5G8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5G8
  • Gene ID64689
  • Target name
    GORASP1
  • Target description
    golgi reassembly stacking protein 1
  • Target synonyms
    GOLPH5, GRASP65, P65, Golgi reassembly-stacking protein 1, Golgi peripheral membrane protein p65, Golgi phosphoprotein 5, Golgi reassembly and stacking protein 1, golgi reassembly stacking protein 1, 65kDa, golgi reassembly-stacking protein of 65 kDa
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9BQQ3
  • Protein Name
    Golgi reassembly-stacking protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GORASP1 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)
  • Reactivity
    Canine, Human, Monkey, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of GRASP65/GORASP1 using anti-GRASP65/GORASP1 antibody (A05896-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRASP65/GORASP1 antigen affinity purified polyclonal antibody (Catalog # A05896-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRASP65/GORASP1 at approximately 65 kDa. The expected band size for GRASP65/GORASP1 is at 65 kDa.
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