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Western Blot analysis of Hela cells using GRB2 Polyclonal Antibody
Western Blot analysis of Hela cells using GRB2 Polyclonal Antibody
Western Blot analysis of Hela cells using GRB2 Polyclonal Antibody

GRB2 Antibody

CSB-PA008951
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGRB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2885
  • Target name
    GRB2
  • Target description
    growth factor receptor bound protein 2
  • Target synonyms
    abundant SRC homology; ASH; EGFRBP-GRB2; epidermal growth factor receptor-binding protein GRB2; epididymis secretory sperm binding protein; Grb3-3; growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; MST084; MSTP084; NCKAP2; protein Ash; SH2/SH3 adapter GRB2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP62993
  • Protein Name
    Growth factor receptor-bound protein 2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (A00351-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # A00351-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25 kDa. The expected band size for GRB2 is at 25 kDa.
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