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GRHL1 Antibody

PACO61726
Assay Genie
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGRHL1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    GRHL1 Antibody (PACO61726)
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID29841
  • Target name
    GRHL1
  • Target description
    grainyhead like transcription factor 1
  • Target synonyms
    LBP32, MGR, NH32, TFCP2L2, grainyhead-like protein 1 homolog, LBP protein 32, grainyhead-like 1, mammalian grainyhead, transcription factor CP2-like 2, transcription factor LBP-32
  • Host
    Rabbit
  • Protein IDQ9NZI5
  • Protein Name
    Grainyhead-like protein 1 homolog
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: GRHL1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71, 50, 58 kDa Observed band size: 71 kDa
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Figure 1. Western blot analysis of Grainyhead-like protein 1/GRHL1 using anti-Grainyhead-like protein 1/GRHL1 antibody (A07368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Grainyhead-like protein 1/GRHL1 antigen affinity purified polyclonal antibody (Catalog # A07368-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Grainyhead-like protein 1/GRHL1 at approximately 70KD. The expected band size for Grainyhead-like protein 1/GRHL1 is at 70KD.
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