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Western Blot analysis of 22RV1 cells using GluR-2 Polyclonal Antibody
Western Blot analysis of 22RV1 cells using GluR-2 Polyclonal Antibody
Western Blot analysis of 22RV1 cells using GluR-2 Polyclonal Antibody

GRIA2 Antibody

CSB-PA002727
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRIA2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRIA2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2891
  • Target name
    GRIA2
  • Target description
    glutamate ionotropic receptor AMPA type subunit 2
  • Target synonyms
    AMPA-selective glutamate receptor 2; GluA2; GLUR2; gluR-2; GLURB; gluR-B; GluR-K2; glutamate receptor 2; glutamate receptor, ionotropic, AMPA 2; HBGR2; NEDLIB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42262
  • Protein Name
    Glutamate receptor 2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRIA2 using anti-GRIA2 antibody (PB9205). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRIA2 antigen affinity purified polyclonal antibody (Catalog # PB9205) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRIA2 at approximately 110KD. The expected band size for GRIA2 is at 99KD.
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WB analysis of extracts from mouse brain tissue using GTX50311 GluR2 (phospho Ser880) antibody. Lane 1 : Primary antibody pre-incubated with the antigen specific peptide
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