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Western Blot Positive WB detected in: Hela whole cell lysate All lanes: GRK3 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: GRK3 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa
Western Blot Positive WB detected in: Hela whole cell lysate All lanes: GRK3 antibody at 2ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa

GRK3 Antibody

CSB-PA001395LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGRK3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRK3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID157
  • Target name
    GRK3
  • Target description
    G protein-coupled receptor kinase 3
  • Target synonyms
    ADRBK2; adrenergic, beta, receptor kinase 2; BARK2; beta-adrenergic receptor kinase 2; beta-ARK-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35626
  • Protein Name
    Beta-adrenergic receptor kinase 2
  • Scientific Description
    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRK3 using anti-GRK3 antibody (A32254-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK3 antigen affinity purified polyclonal antibody (Catalog # A32254-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRK3 at approximately 80 kDa. The expected band size for GRK3 is at 80 kDa.
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