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ProductsBack/Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAS7 (Cat# RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7(Cat# TA501907). Positive lysates [LY418527] (100ug) and [LC418527] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAS7 (Cat# RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7(Cat# TA501907). Positive lysates [LY418527] (100ug) and [LC418527] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GAS7 (Cat# RC215256, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GAS7(Cat# TA501907). Positive lysates [LY418527] (100ug) and [LC418527] (20ug) can be purchased separately from OriGene.

Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10]

CF501907
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGAS7
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Growth Arrest Specific Protein 7 (GAS7) Mouse Monoclonal Antibody [Clone ID: OTI8H10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI8H10
  • Gene ID8522
  • Target name
    GAS7
  • Target description
    growth arrest specific 7
  • Target synonyms
    growth arrest-specific protein 7
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDO60861
  • Protein Name
    Growth arrest-specific protein 7
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GAS7 mouse monoclonal antibody, clone OTI8H10 (formerly 8H10)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GAS7 using anti-GAS7 antibody (A06548-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAS7 antigen affinity purified polyclonal antibody (Catalog # A06548-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAS7 at approximately 50 kDa. The expected band size for GAS7 is at 54 kDa.
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