
Grp78 antibody detects Grp78 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Grp78 antibody (GTX102567) dilution: 1:1000
Grp78 antibody [C2C3], C-term
GTX102567
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetHSPA5
Overview
- SupplierGeneTex
- Product NameGrp78 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3309
- Target nameHSPA5
- Target descriptionheat shock protein family A (Hsp70) member 5
- Target synonymsBIP, GRP78, HEL-S-89n, endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, HSP70 family protein 5, binding-immunoglobulin protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, epididymis secretory sperm binding protein Li 89n, glucose-regulated protein, 78kDa, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), heat shock protein 70 family protein 5, heat shock protein family A member 5, immunoglobulin heavy chain-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP11021
- Protein NameEndoplasmic reticulum chaperone BiP
- Scientific DescriptionWhen Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as immunoglobulin heavy chain-binding protein (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161

![Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [C2C3], C-term (GTX102567) diluted at 1:1000. Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [C2C3], C-term (GTX102567) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102567/GTX102567_39680_20160728_WB_shRNA_watermark_w_23060100_446.webp)






![Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221118_IHC-P_R_22112219_613.webp)