Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221118_IHC-P_M_22112219_111.webp "Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221202_ICC_IF_22120719_777.webp "Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221202_WB_M_R_22120719_664.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_44914_20230113_WB_TPM_watermark_23011618_484.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_44914_20230421_WB_multiple_B_23042500_276.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) HepG2 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_44914_20230804_WB_shRNA_watermark_23080901_880.webp "Non-transfected (–) and transfected (+) HepG2 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp78 antibody [HL1815] (GTX637540) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Grp78 antibody [HL1815]
GTX637540
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPA5
Overview
- SupplierGeneTex
- Product NameGrp78 antibody [HL1815]
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1815
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3309
- Target nameHSPA5
- Target descriptionheat shock protein family A (Hsp70) member 5
- Target synonymsBIP, GRP78, HEL-S-89n, endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, HSP70 family protein 5, binding-immunoglobulin protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, epididymis secretory sperm binding protein Li 89n, glucose-regulated protein, 78kDa, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), heat shock protein 70 family protein 5, heat shock protein family A member 5, immunoglobulin heavy chain-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP11021
- Protein NameEndoplasmic reticulum chaperone BiP
- Scientific DescriptionThe protein encoded by this gene is a member of the heat shock protein 70 (HSP70) family. It is localized in the lumen of the endoplasmic reticulum (ER), and is involved in the folding and assembly of proteins in the ER. As this protein interacts with many ER proteins, it may play a key role in monitoring protein transport through the cell.[provided by RefSeq, Sep 2010]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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