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GRSF1 Antibody (YA2955, Rabbit)

HY-P83210
MedChem Express
TargetGRSF1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    GRSF1 Antibody (YA2955, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2926
  • Target name
    GRSF1
  • Target description
    G-rich RNA sequence binding factor 1
  • Target synonyms
    G-rich sequence factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    GRSF1 Antibody (YA2955) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2955), targeting GRSF1, with a predicted molecular weight of 53 kDa (observed band size: 53 kDa). GRSF1 Antibody (YA2955) can be used for WB, ICC/IF experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRSF1 using anti-GRSF1 antibody (A07576-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRSF1 antigen affinity purified polyclonal antibody (Catalog # A07576-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRSF1 at approximately 53KD. The expected band size for GRSF1 is at 53KD.
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