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Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-GSTA3 Polyclonal Antibody, Unconjugated (bs-5163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-GSTA3 Polyclonal Antibody, Unconjugated (bs-5163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse brain labeled with Rabbit Anti-GSTA3 Polyclonal Antibody, Unconjugated (bs-5163R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

GSTA3 Polyclonal Antibody

BS-5163R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetGSTA3
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    GSTA3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2940
  • Target name
    GSTA3
  • Target description
    glutathione S-transferase alpha 3
  • Target synonyms
    glutathione S-alkyltransferase A3; glutathione S-aralkyltransferase A3; glutathione S-aryltransferase A3; glutathione S-transferase A3; glutathione S-transferase A3-3; GST class-alpha member 3; GSTA3-3; GTA3; S-(hydroxyalkyl)glutathione lyase A3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16772
  • Protein Name
    Glutathione S-transferase A3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • PCB52 induces hepatotoxicity in male offspring through aggravating loss of clearance capacity and activating the apoptosis: Sex-biased effects on rats. Xie XL et al., 2019 Jul, Chemosphere
    Read more
  • Reduction of Aflatoxin B1 Toxicity by Lactobacillus plantarum C88: A Potential Probiotic Strain Isolated from Chinese Traditional Fermented Food Tofu. Huang L et al., 2017, PLoS One
    Read more

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Figure 1. Western blot analysis of Gsta3 using anti-Gsta3 antibody (A05829-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: rat ovary tissue lysates, Lane 3: rat brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gsta3 antigen affinity purified polyclonal antibody (Catalog # A05829-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gsta3 at approximately 28KD. The expected band size for Gsta3 is at 28KD.
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