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Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: GSTM3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 27 KDa Observed band size: 27 KDa Exposure time : 10min
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: GSTM3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 27 KDa Observed band size: 27 KDa Exposure time : 10min
Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate All lanes: GSTM3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 27 KDa Observed band size: 27 KDa Exposure time : 10min

GSTM3 Monoclonal Antibody

CSB-MA009982A0M
Cusabio
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGSTM3
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Overview

  • Supplier
    Cusabio
  • Product Name
    GSTM3 Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1G1F1
  • Conjugate
    Unconjugated
  • Gene ID2947
  • Target name
    GSTM3
  • Target description
    glutathione S-transferase mu 3
  • Target synonyms
    GST5, GSTB, GSTM3-3, GSTM3TV2, GTM3, hGSTM3-3, glutathione S-transferase Mu 3, GST class-mu 3, S-(hydroxyalkyl)glutathione lyase M3, brain GST, brain type mu-glutathione S-transferase, epididymis secretory sperm binding protein, glutathione S-alkyltransferase M3, glutathione S-aralkyltransferase M3, glutathione S-aryltransferase M3, glutathione S-transferase M3 (brain), glutathione S-transferase mu 3 (brain), glutathione S-transferase, Mu-3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP21266
  • Protein Name
    Glutathione S-transferase Mu 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Mouse kidney tissue All lanes: GSTM3 antibody at 2.8ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 27 kDa Observed band size: 27 kDa
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Figure 1. Western blot analysis of GSTM3 using anti-GSTM3 antibody (A03298-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat testis tissue lysate, Lane 2: rat brain tissue lysate, Lane 3: mouse testis tissue lysate, Lane 4: mouse brain tissue lysate, Lane 5: human Hela cell lysate, Lane 6: human MCF-7 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTM3 antigen affinity purified polyclonal antibody (Catalog # A03298-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTM3 at approximately 26KD. The expected band size for GSTM3 is at 26KD.
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