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GSTT1 Antibody

LS-C749011
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetGSTT1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GSTT1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 13kDa/27kDa, while the observed MW by Western blot was 27kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 13kDa/27kDa, while the observed MW by Western blot was 27kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2952
  • Target name
    GSTT1
  • Target description
    glutathione S-transferase theta 1
  • Target synonyms
    glutathione S-transferase theta-1; glutathione transferase T1-1; GST class-theta-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GSTT1 using anti-GSTT1 antibody (A01084). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat lung tissue lysates, Lane 3: mouse liver tissue lysates, Lane 4: mouse lung tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTT1 antigen affinity purified polyclonal antibody (Catalog # A01084) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTT1 at approximately 27KD. The expected band size for GSTT1 is at 27KD.
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