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Western Blot All lanes: GTF2F2 antibody at 16microg/ml Lane 1: Mouse spleen tissue Lane 2: K562 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Western Blot All lanes: GTF2F2 antibody at 16microg/ml Lane 1: Mouse spleen tissue Lane 2: K562 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa
Western Blot All lanes: GTF2F2 antibody at 16microg/ml Lane 1: Mouse spleen tissue Lane 2: K562 whole cell lysate Lane 3: A549 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 29 kDa Observed band size: 29 kDa

GTF2F2 Antibody

CSB-PA010005LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetGTF2F2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GTF2F2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2963
  • Target name
    GTF2F2
  • Target description
    general transcription factor IIF subunit 2
  • Target synonyms
    ATP-dependent helicase GTF2F2; BTF4; general transcription factor IIF 30 kDa subunit; general transcription factor IIF subunit 2; general transcription factor IIF, polypeptide 2, 30kDa; RAP30; TF2F2; TFIIF; TFIIF-beta; transcription initiation factor IIF subunit beta; transcription initiation factor RAP30
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13984
  • Protein Name
    General transcription factor IIF subunit 2
  • Scientific Description
    TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GTF2F2 using anti-GTF2F2 antibody (A10896-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GTF2F2 antigen affinity purified polyclonal antibody (Catalog # A10896-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GTF2F2 at approximately 28 kDa. The expected band size for GTF2F2 is at 28 kDa.
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