ELISA results of purified Donkey Anti-Guinea Pig IgG Antibody Min X10 Serums tested against purified Guinea Pig IgG. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Gt-a-Dky IgG HRP conjugate (p/n 616-1302), and TMB substrate (p/n TMBE-1000).