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Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HAGH(GTX105708) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HAGH(GTX105708) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using HAGH(GTX105708) antibody at 1:100 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

HAGH antibody [N2C3]

GTX105708
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetHAGH
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Overview

  • Supplier
    GeneTex
  • Product Name
    HAGH antibody [N2C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3029
  • Target name
    HAGH
  • Target description
    hydroxyacylglutathione hydrolase
  • Target synonyms
    GLO2, GLO2D, GLX2, GLXII, HAGH1, hydroxyacylglutathione hydrolase, mitochondrial, glyoxalase II, hydroxyacylglutathione hydroxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16775
  • Protein Name
    Hydroxyacylglutathione hydrolase, mitochondrial
  • Scientific Description
    The enzyme encoded by this gene is classified as a thiolesterase and is responsible for the hydrolysis of S-lactoyl-glutathione to reduced glutathione and D-lactate. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HAGH using anti-HAGH antibody (A08943-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAGH antigen affinity purified polyclonal antibody (Catalog # A08943-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAGH at approximately 29 kDa. The expected band size for HAGH is at 34 kDa.
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