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Haptoglobin antibody

GTX13431
GeneTex
ApplicationsImmunoDiffusion, ELISA, Other Application
Product group Antibodies
ReactivityHuman
TargetHP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Haptoglobin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    ELISA: 1:30,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoDiffusion, ELISA, Other Application
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3240
  • Target name
    HP
  • Target description
    haptoglobin
  • Target synonyms
    BP, HP2ALPHA2, HPA1S, haptoglobin, binding peptide, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin, alpha polypeptide, haptoglobin, beta polypeptide, zonulin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00738
  • Protein Name
    Haptoglobin
  • Scientific Description
    Haptoglobin combines with free plasma hemoglobin, prevent loss of iron through the kidneys and protect the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes.
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HP using anti-HP antibody (A00062-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HP antigen affinity purified polyclonal antibody (Catalog # A00062-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HP at approximately 45KD. The expected band size for HP is at 45KD.
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