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ProductsBack/HAUS7 Antibody (aa318-368)

HAUS7 Antibody (aa318-368)

LS-C763070
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetHAUS7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HAUS7 Antibody (aa318-368)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:2000 - 1:10000) Applications should be user optimized. IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55559
  • Target name
    HAUS7
  • Target description
    HAUS augmin like complex subunit 7
  • Target synonyms
    26S proteasome-associated UCH37-interacting protein 1; HAUS augmin-like complex subunit 7; UCHL5IP; UIP1; X-linked protein STS1769
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UCHL5IP/HAUS7 using anti-UCHL5IP/HAUS7 antibody (A12338-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL5IP/HAUS7 antigen affinity purified polyclonal antibody (Catalog # A12338-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCHL5IP/HAUS7 at approximately 46 kDa. The expected band size for UCHL5IP/HAUS7 is at 41 kDa.
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