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Western Blot analysis of k562 cell and Hela cell using HAVCR1 Polyclonal Antibody at dilution of 1:700
Western Blot analysis of k562 cell and Hela cell using HAVCR1 Polyclonal Antibody at dilution of 1:700
Western Blot analysis of k562 cell and Hela cell using HAVCR1 Polyclonal Antibody at dilution of 1:700

HAVCR1 Polyclonal Antibody

E-AB-15501
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HAVCR1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    The protein encoded by this gene is a membrane receptor for both human hepatitis A virus (HHAV) and TIMD4. The encoded protein may be involved in the moderation of asthma and allergic diseases. The reference genome represents an allele that retains a MTTVP amino acid segment that confers protection against atopy in HHAV seropositive individuals. Three transcript variants encoding the same protein have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TIM 1 using anti-TIM 1 antibody (A01306). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: recombinant human TIM1 protein 1ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TIM 1 antigen affinity purified polyclonal antibody (Catalog # A01306) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TIM 1 at approximately 39KD.
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