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HBA1 Antibody (aa1-142, FITC)

LS-C422881
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
TargetHBA1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HBA1 Antibody (aa1-142, FITC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications and images are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. ICC (1:50 - 1:500), WB (1:50 - 1:400) Applications and images are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    FITC
  • Estimated Purity
    ...
  • Gene ID3039
  • Target name
    HBA1
  • Target description
    hemoglobin subunit alpha 1
  • Target synonyms
    alpha globin chain; alpha one globin; alpha-2 globin chain; delta globin; ECYT7; HBA-T3; HBH; hemoglobin alpha 1 globin chain; hemoglobin subunit alpha; hemoglobin, alpha 1; METHBA
  • Host
    Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hemoglobin using anti-Hemoglobin antibody (A00233-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemoglobin antigen affinity purified polyclonal antibody (Catalog # A00233-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemoglobin at approximately 15 kDa. The expected band size for Hemoglobin is at 15 kDa.
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