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Western blot All lanes: Hemoglobin polyclonal antibody at 2microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Western blot All lanes: Hemoglobin polyclonal antibody at 2microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa
Western blot All lanes: Hemoglobin polyclonal antibody at 2microg/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 16 kDa Observed band size: 16 kDa

Hemoglobin Antibody

CSB-PA00240E0RB
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
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Overview

  • Supplier
    Cusabio
  • Product Name
    Hemoglobin Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues. The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Hemoglobin using anti-Hemoglobin antibody (A00233-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hemoglobin antigen affinity purified polyclonal antibody (Catalog # A00233-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hemoglobin at approximately 15 kDa. The expected band size for Hemoglobin is at 15 kDa.
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