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HCG-alpha(HCGa/53), CF405S conjugate, 0.1mg/mL [26628-22-8]

HCG-alpha(HCGa/53), CF405S conjugate, 0.1mg/mL [26628-22-8]

BNC040053
Biotium
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCGA
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Overview

  • Supplier
    Biotium
  • Product Name
    HCG-alpha(HCGa/53), CF405S conjugate, 0.1mg/mL
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HCGa/53
  • Concentration
    0.1 mg/ml
  • Conjugate
    Other Conjugate
  • Gene ID1081
  • Target name
    CGA
  • Target description
    glycoprotein hormones, alpha polypeptide
  • Target synonyms
    anterior pituitary glycoprotein hormones common subunit alpha; CG-ALPHA; choriogonadotropin alpha chain; chorionic gonadotrophin subunit alpha; chorionic gonadotropin, alpha polypeptide; follicle-stimulating hormone alpha chain; follicle-stimulating hormone alpha subunit; follitropin alpha chain; FSHA; FSH-alpha; glycoprotein hormones alpha chain; GPA1; GPHa; GPHA1; HCG; LHA; LSH-alpha; luteinizing hormone alpha chain; lutropin alpha chain; thyroid-stimulating hormone alpha chain; thyrotropin alpha chain; TSHA; TSH-alpha
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP01215
  • Protein Name
    Glycoprotein hormones alpha chain
  • Scientific Description
    This MAb reacts with a protein of ~13 kDa, identified as alpha sub-unit of HCG. HCG is a glycoprotein, which is secreted in large quantities by normal trophoblasts. It is present only in trace amounts in non-pregnant urine and sera but rises sharply during pregnancy. HCG is composed of two non-identical, non-covalently linked polypeptide chains designated as the alpha and beta subunits. The alpha subunit is identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH), and luteinizing hormone (LH).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
  • Source
    Animal
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Chromogranin A/CGA using anti-Chromogranin A/CGA antibody (A00739-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Chromogranin A/CGA antigen affinity purified polyclonal antibody (Catalog # A00739-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Chromogranin A/CGA at approximately 17 kDa. The expected band size for Chromogranin A/CGA is at 13 kDa.
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