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Immunohistochemistry of paraffin-embedded Human breast cancer tissue using HCN2 Polyclonal Antibody at dilution 1:80
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using HCN2 Polyclonal Antibody at dilution 1:80
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using HCN2 Polyclonal Antibody at dilution 1:80

HCN2 Polyclonal Antibody

E-AB-15709
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HCN2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.6mg/ml
  • Scientific Description
    Hyperpolarization-activated cation channels of the HCN gene family, such as HCN2, contribute to spontaneous rhythmic activity in both heart and brain. Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages.
  • UNSPSC
    12352203

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Figure 2. IHC analysis of HCN2 using anti-HCN2 antibody (A02804). HCN2 was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-HCN2 Antibody (A02804) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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