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WB analysis of HeLa cell lysate using GTX30496 HDAC10 antibody. Dilution : 1:250
WB analysis of HeLa cell lysate using GTX30496 HDAC10 antibody. Dilution : 1:250
WB analysis of HeLa cell lysate using GTX30496 HDAC10 antibody. Dilution : 1:250

HDAC10 antibody

GTX30496
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetHDAC10
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Overview

  • Supplier
    GeneTex
  • Product Name
    HDAC10 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID83933
  • Target name
    HDAC10
  • Target description
    histone deacetylase 10
  • Target synonyms
    HD10, polyamine deacetylase HDAC10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ969S8
  • Protein Name
    Histone deacetylase 10
  • Scientific Description
    Acetylation of histone core particles modulates chromatin structure and gene expression. The opposing enzymatic activities of histone acetyltransferases and histone deacetylases, such as HDAC10, determine the acetylation status of histone tails (Kao et al., 2002 [PubMed 11677242]).[supplied by OMIM]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC10 using anti-HDAC10 antibody (A05215-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC10 antigen affinity purified polyclonal antibody (Catalog # A05215-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC10 at approximately 71KD. The expected band size for HDAC10 is at 71KD.
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