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HDAC11 Recombinant Antibody

BSM-61104R
Bioss Antibodies
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetHDAC11
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    HDAC11 Recombinant Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    WB(1:300-5000), IP(1-2ug)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID79885
  • Target name
    HDAC11
  • Target description
    histone deacetylase 11
  • Target synonyms
    HD11; histone deacetylase 11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96DB2
  • Protein Name
    Histone deacetylase 11
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: HDAC11 antibody at 2ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 40, 34 kDa Observed band size: 40 kDa
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Figure 1. Western blot analysis of HDAC11 using anti-HDAC11 antibody (PB9630). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse brain tissue lysates, Lane 5: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC11 antigen affinity purified polyclonal antibody (Catalog # PB9630) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC11 at approximately 37 kDa. The expected band size for HDAC11 is at 39 kDa.
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