Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4(GTX110231) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110231/GTX110231_42795_20181221_WB_M_w_23060500_150.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110231/GTX110231_43754_20191101_WB_w_23060500_277.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110231/GTX110231_42795_20181221_WB_R_w_23060500_137.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX110231/GTX110231_42795_20181026_WB_shRNA_watermark_w_23060500_628.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal (GTX110231) 5 % SDS-PAGE The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]](https://www.genetex.com/upload/website/prouct_img/normal/GTX110231/GTX110231_40478_IP_w_23060500_315.webp "HDAC4 antibody [N3C1], Internal immunoprecipitates HDAC4 protein in IP experiments. IP samples: Jurkat whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of HDAC4 protein by 4 μg HDAC4 antibody [N3C1], Internal (GTX110231) 5 % SDS-PAGE The immunoprecipitated HDAC4 protein was detected by HDAC4 antibody [N3C1], Internal (GTX110231) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]")
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HDAC4(GTX110231) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
HDAC4 antibody [N3C1], Internal
GTX110231
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC4
Overview
- SupplierGeneTex
- Product NameHDAC4 antibody [N3C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration2.1 mg/ml
- ConjugateUnconjugated
- Gene ID9759
- Target nameHDAC4
- Target descriptionhistone deacetylase 4
- Target synonymsAHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID, histone deacetylase 4, histone deacetylase A
- HostRabbit
- IsotypeIgG
- Protein IDP56524
- Protein NameHistone deacetylase 4
- Scientific DescriptionHistones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhao B, Fu J, Ni H, et al. Catalpol ameliorates CFA-induced inflammatory pain by targeting spinal cord and peripheral inflammation. Front Pharmacol. 2022,13:1010483. doi: 10.3389/fphar.2022.1010483Read this paper
- Lai CY, Hsieh MC, Ho YC, et al. GluN2B/CaMKII mediates CFA-induced hyperalgesia via HDAC4-modified spinal COX2 transcription. Neuropharmacology. 2018,135:536-546. doi: 10.1016/j.neuropharm.2018.03.012Read this paper
- Lin TB, Hsieh MC, Lai CY, et al. Melatonin relieves neuropathic allodynia through spinal MT2-enhanced PP2Ac and downstream HDAC4 shuttling-dependent epigenetic modification of hmgb1 transcription. J Pineal Res. 2016,60(3):263-76. doi: 10.1111/jpi.12307Read this paper
- Lin TB, Hsieh MC, Lai CY, et al. Modulation of Nerve Injury-induced HDAC4 Cytoplasmic Retention Contributes to Neuropathic Pain in Rats. Anesthesiology. 2015,123(1):199-212. doi: 10.1097/ALN.0000000000000663Read this paper
Datasheet
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