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Western Blot analysis of HepG2 cells using HDAC6 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HDAC6 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HDAC6 Polyclonal Antibody

HDAC6 Antibody

CSB-PA002895
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHDAC6
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10013
  • Target name
    HDAC6
  • Target description
    histone deacetylase 6
  • Target synonyms
    CPBHM, HD6, JM21, KDAC6, PPP1R90, protein deacetylase HDAC6, E3 ubiquitin-protein ligase HDAC6, alpha-tubulin deacetylase HDAC6, protein phosphatase 1, regulatory subunit 90, tubulin-lysine deacetylase HDAC6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UBN7
  • Protein Name
    Histone deacetylase 6
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDAC6 using anti-HDAC6 antibody (PB9628). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC6 antigen affinity purified polyclonal antibody (Catalog # PB9628) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC6 at approximately 160 kDa. The expected band size for HDAC6 is at 131 kDa.
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