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WB analysis of mouse testis tissue lysate using GTX33237 HDGFRP2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse testis tissue lysate using GTX33237 HDGFRP2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of mouse testis tissue lysate using GTX33237 HDGFRP2 antibody. Dilution : 1:1000 Loading : 25microg per lane

HDGFRP2 antibody

GTX33237
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHDGFL2
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Overview

  • Supplier
    GeneTex
  • Product Name
    HDGFRP2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID84717
  • Target name
    HDGFL2
  • Target description
    HDGF like 2
  • Target synonyms
    HDGF2; HDGF-2; HDGF-related protein 2; HDGFRP2; hepatoma-derived growth factor-related protein 2; HRP2; HRP-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z4V5
  • Protein Name
    Hepatoma-derived growth factor-related protein 2
  • Scientific Description
    This gene encodes a member of the hepatoma-derived growth factor (HDGF) family. The protein includes an N-terminal PWWP domain that binds to methyl-lysine-containing histones, with specific binding of this protein to tri-methylated lysines 36 and 79 of histone H3, and di- and tri-methylated lysine 20 of histone H4. The protein functions in LEDGF/p75-independent HIV-1 replication by determining HIV-1 integration site selection. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDGFL2 using anti-HDGFL2 antibody (A32401-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDGFL2 antigen affinity purified polyclonal antibody (Catalog # A32401-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDGFL2 at approximately 140 kDa. The expected band size for HDGFL2 is at 74 kDa.
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