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ProductsBack/HDHD2 Mouse Monoclonal Antibody [Clone ID: OTI2C12]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD2 (RC205967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD2. Positive lysates LY403144 (100ug) and LC403144 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD2 (RC205967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD2. Positive lysates LY403144 (100ug) and LC403144 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDHD2 (RC205967, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDHD2. Positive lysates LY403144 (100ug) and LC403144 (20ug) can be purchased separately from OriGene.

HDHD2 Mouse Monoclonal Antibody [Clone ID: OTI2C12]

CF500914
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDHD2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HDHD2 Mouse Monoclonal Antibody [Clone ID: OTI2C12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2C12
  • Gene ID84064
  • Target name
    HDHD2
  • Target description
    haloacid dehalogenase like hydrolase domain containing 2
  • Target synonyms
    3110052N05Rik, HEL-S-301, haloacid dehalogenase-like hydrolase domain-containing protein 2, epididymis secretory protein Li 301, epididymis secretory sperm binding protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9H0R4
  • Protein Name
    Haloacid dehalogenase-like hydrolase domain-containing protein 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HDHD2 mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDHD2 using anti-HDHD2 antibody (A15632-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat kidney tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse kidney tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDHD2 antigen affinity purified polyclonal antibody (Catalog # A15632-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDHD2 at approximately 28 kDa. The expected band size for HDHD2 is at 29 kDa.
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