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Immunohistochemistry of paraffin-embedded Rat liver using HDLBP Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat liver using HDLBP Polyclonal Antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded Rat liver using HDLBP Polyclonal Antibody at dilution of 1:100 (40x lens).

HDLBP Polyclonal Antibody

E-AB-65527
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HDLBP Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene binds high density lipoprotein (HDL) and may function to regulate excess cholesterol levels in cells. The encoded protein also binds RNA and can induce heterochromatin formation.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDLBP using anti-HDLBP antibody (A06727-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDLBP antigen affinity purified polyclonal antibody (Catalog # A06727-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDLBP at approximately 150 kDa. The expected band size for HDLBP is at 141 kDa.
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Lane 1: Mouse Serum Lysates. Probed with High density lipoprotein polyclonal Antibody, unconjugated (bs-4589R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using CSB-PA211136(HDLBP Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: x200)
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