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ProductsBack/Heat Shock Protein 27 (Hsp27) Polyclonal Antibody

Heat Shock Protein 27 (Hsp27) Polyclonal Antibody

CAU27344
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse
TargetHspb1
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Overview

  • Supplier
    Biomatik
  • Product Name
    Heat Shock Protein 27 (Hsp27) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID15507
  • Target name
    Hspb1
  • Target description
    heat shock protein 1
  • Target synonyms
    27kDa, Hsp25, heat shock protein beta-1, HSP 25, HSP 27, growth-related 25 kDa protein, heat shock 25 kDa protein, heat shock 27 kDa protein, heat shock 27kDa protein 1, heat shock protein, 25 kDa, p25
  • Host
    Rabbit
  • Protein IDP14602
  • Protein Name
    Heat shock protein beta-1
  • Scientific Description
    The Heat Shock Protein 27 (Hsp27) Polyclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity
    Mouse
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Hsp27 using anti-Hsp27 antibody (A00676). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Hsp27 antigen affinity purified polyclonal antibody (Catalog # A00676) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Hsp27 at approximately 27 kDa. The expected band size for Hsp27 is at 23 kDa.
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