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HECTD1 Antibody

CAB9433
Assay Genie
TargetHECTD1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    HECTD1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID25831
  • Target name
    HECTD1
  • Target description
    HECT domain E3 ubiquitin protein ligase 1
  • Target synonyms
    EULIR, E3 ubiquitin-protein ligase HECTD1, E3 ligase for inhibin receptor, HECT domain containing E3 ubiquitin protein ligase 1, HECT domain-containing protein 1, HECT-type E3 ubiquitin transferase HECTD1
  • Host
    Rabbit
  • Protein IDQ9ULT8
  • Protein Name
    E3 ubiquitin-protein ligase HECTD1
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HECTD1 using anti-HECTD1 antibody (A08428-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HECTD1 antigen affinity purified polyclonal antibody (Catalog # A08428-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HECTD1 at approximately
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