More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HECTD3 Antibody
Immunofluorescence staining of A549 cells with CSB-PA725567LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cells with CSB-PA725567LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cells with CSB-PA725567LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

HECTD3 Antibody

CSB-PA725567LA01HU
Cusabio
ApplicationsImmunoFluorescence, ELISA
Product group Antibodies
ReactivityHuman
TargetHECTD3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    HECTD3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79654
  • Target name
    HECTD3
  • Target description
    HECT domain E3 ubiquitin protein ligase 3
  • Target synonyms
    E3 ubiquitin-protein ligase HECTD3, HECT domain containing E3 ubiquitin protein ligase 3, HECT-type E3 ubiquitin transferase HECTD3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5T447
  • Protein Name
    E3 ubiquitin-protein ligase HECTD3
  • Scientific Description
    E3 ubiquitin ligases accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of TRIOBP and its subsequent proteasomal degradation, thus faciliting cell cycle progression by regulating the turn-over of TRIOBP. Mediates also ubiquitination of STX8 (By similarity).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of HECTD3 using anti-HECTD3 antibody (A11560). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HECTD3 antigen affinity purified polyclonal antibody (Catalog # A11560) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HECTD3 at approximately 97KD. The expected band size for HECTD3 is at 97KD.
Product group Antibodies
Boster Bio
Anti-HECTD3 Antibody Picoband(r)A11560-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
TargetHECTD3
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HECTD3 Antibody (aa811-861)LS-C762444
ApplicationsImmunoPrecipitation, Western Blot
TargetHECTD3
  • SizePrice
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-HECTD3 AntibodyHPA027467
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetHECTD3
  • SizePrice